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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) Multiple sources of infection with unrelated Salmonella strains.
B) A common ancestral strain that has undergone flagellar antigen variation.
C) Contamination in the laboratory affecting multiple samples.
D) A single point source of infection with a genetically stable strain.

2. A Klebsiella pneumoniae isolate from a bloodstream infection shows the following susceptibility profile:
Meropenem (MIC = 8 µg/mL, R), Imipenem (MIC = 16 µg/mL, R), Ertapenem (MIC = 4 µg/mL, R), Ceftazidime/avibactam (MIC = 1 µg/mL, S), Aztreonam (MIC = 32 µg/mL, R). Which resistance mechanism is most strongly suggested by this pattern?

A) Extended-Spectrum Beta-Lactamase (ESBL) production alone
B) Klebsiella pneumoniae Carbapenemase (KPC) production
C) Metallo-beta-lactamase (MBL) production
D) AmpC beta-lactamase hyperproduction coupled with porin loss

3. A patient with a history of recurrent sinusitis and bronchitis is diagnosed with cystic fibrosis. Sputum cultures repeatedly grow a Gram-negative rod that is oxidase-positive and produces a blue-green pigment. This organism's chronic colonization of the respiratory tract in cystic fibrosis patients is primarily due to its ability to:

A) Exhibit rapid intracellular replication within respiratory epithelial cells.
B) Produce potent exotoxins that damage the ciliated epithelium.
C) Evade phagocytosis by alveolar macrophages through capsule production.
D) Form a biofilm that protects it from host defenses and antibiotics.

4. An immunocompromised patient returning from a trip to the southwestern United States presents with fever, cough, and chest pain. Chest X-ray shows diffuse pulmonary infiltrates. Sputum culture grows a dimorphic fungus that forms spherules containing endospores at 37°C. The MOST likely causative agent is:

A) Paracoccidioides brasiliensis
B) Coccidioides immitis
C) Blastomyces dermatitidis
D) Histoplasma capsulatum

5. A patient with a history of liver transplantation develops a bloodstream infection with a Gram-negative bacterium. The isolate is identified as Sphingomonas paucimobilis. This opportunistic pathogen is known for its ability to:

A) Degrade a wide range of environmental pollutants.
B) Produce potent exotoxins that directly damage host tissues.
C) Form biofilms on indwelling catheters and prosthetic devices.
D) Exhibit intrinsic resistance to a broad spectrum of antibiotics.

Solutions: