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American Society of Microbiology ABMM Sample Questions:

1. What is considered the "gold standard" for routine monitoring of the lethality of steam sterilization (autoclave) cycles in healthcare facilities?

A) Regular use of biological indicators containing spores of Geobacillus stearothermophilus.
B) Daily check of autoclave cycle printouts for correct time, temperature, and pressure.
C) Use of chemical indicator strips placed inside each sterilized pack.
D) Performing a Bowie-Dick test at the beginning of each day for pre-vacuum sterilizers.

2. A clinical microbiology laboratory is evaluating a new rapid diagnostic test for influenza viruses. The test has a high sensitivity but a lower specificity compared to RT-PCR. In a setting with a low prevalence of influenza, the use of this rapid test is MOST likely to result in a high number of:

A) False positive results
B) False negative results
C) True positive results
D) True negative results

3. An immunocompromised patient develops a disseminated fungal infection. Tissue biopsy reveals small (2-4
µm) yeast cells within macrophages. The patient has a history of living in the Ohio and Mississippi River valleys. The MOST likely causative agent is:

A) Paracoccidioides brasiliensis
B) Blastomyces dermatitidis
C) Penicillium marneffei
D) Histoplasma capsulatum

4. A patient develops severe, watery diarrhea shortly after starting a course of clindamycin. Stool testing using a cytotoxicity assay is positive. This result is most consistent with infection by which organism?

A) Giardia lamblia
B) Campylobacter jejuni
C) Norovirus
D) Clostridioides difficile

5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate using broth microdilution. The lowest concentration of antibiotic that inhibits visible growth of the organism is reported as the:

A) Minimum inhibitory concentration (MIC)
B) Minimum bactericidal concentration (MBC)
C) Zone diameter
D) Breakpoint

Solutions: