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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Microbiology | 15-20% | - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology - Identification of pathogenic microorganisms |
| Topic 2: Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Physical, chemical, and microscopic analysis |
| Topic 3: Immunology and Serology | 5-10% | - Infectious disease serology - Immune system principles and components - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies |
| Topic 4: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests |
| Topic 5: Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures - Red blood cell physiology and disorders |
| Topic 6: Blood Banking (Immunohematology) | 15-20% | - Antibody detection and identification - Transfusion reactions and adverse events - Blood group systems and antigens - Compatibility testing - Blood components and storage |
| Topic 7: Laboratory Operations | 5-10% | - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Which of the following disorders is characterized by increased production of chloride in sweat?
Multiple myeloma
- A. Cystic fibrosis
- B. Hypoparathyoidism
- C. Wilson's disease
- D. Down Syndrome
Correct Answer: A 🗳️
The National Heart, Lung, and Blood Institute initiated the National Cholesterol Education Program (NCEP) in 1985. The goal was to reduce the number of Americans with elevated cholesterol and thus reduce illnesses and deaths in the United States due to coronary heart disease. Three adult treatment panels have been published since then with clinical practice guidelines for managing cholesterol levels in adults.
The most recent panel, Adult Treatment Panel III (ATP III), was published in 2001 and updated in 2004. The NCEP: ATP III also includes criteria for the diagnosis of metabolic syndrome.
Select the set of laboratory assays that are utilized in the NCEP: ATP III criteria for metabolic syndrome diagnosis.
- A. LDL-C, triglycerides, HDL-C, and fasting blood glucose
- B. Fasting blood glucose, triglycerides, insulin, and VLDL
- C. Fasting blood glucose, triglycerides, HDL-C
- D. Fasting blood glucose, triglycerides, HDL-C, and VLDL
Correct Answer: C 🗳️
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following?
- A. Chronic
- B. Mantle cell lymphoma
- C. A normal B cell
- D. A mature T cell
Correct Answer: A 🗳️
Which of the following CD markers is NOT present on a normal mature T cell?
- A. CD5
- B. CD7
- C. CD20
- D. CD3
Correct Answer: C 🗳️
A casual blood glucose >/= 200 mg/dL on a patient with symptoms and an A1C >/= 6.5% meet diagnostic criteria for diabetes.
Chemistry
A physician is evaluating a 45-year-old obese male for diabetes and orders a plasma glucose at time of evaluation and a HbA1C one week later. The patient has a family history of diabetes and currently exhibits symptoms of diabetes.
What would be the best course of action if these are the blood glucose results?
Casual Blood Glucose: 208 mg/dL
HbA1C one week later: 7.2%
- A. Order a C-peptide for diagnosis
- B. Patient meets criteria for diagnosis of diabetes
- C. Order insulin levels; if decreased, diagnose diabetes
- D. The patient is at risk for diabetes (pre-diabetic); monitor for diabetes with yearly FPG levels
Correct Answer: B 🗳️
Deborah -
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